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Reticulocytes Procedure

 Reticulocytes are immature red blood cells normally present in small numbers in the blood (up to 2%) Reticulocyte numbers increase when there is increase in erythropoitic activity. A Reticulocyte count asses bone marrow activity, e.g. whether there is an effective erythropoitic response when there is reduction in the number of the red blood cells due to haemolysis or hemorrhage. A Reticulocyte count   also of value in monitoring the erythropoitic response of an anemic patient.

Procedure 

• Pour 2-3 drops of blood with EDTA 

• Add 2-3 drops of stain 10 g/l (1% w/v) new methylene blue in same tube and mix well

• Incubate for 20 minutes at room temperature 37 degree centigrade

• Mix gently and take one drop with dropper and pour on the slide and make a smear and leave to dry

• Now smear is ready to observe in microscope for reticulates count

Result

Reticulocyte appears as Pale green-blue stained cells containing dark blue- violet inclusion in the form of small granules.


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