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Antinuclear Antibodies Procedure

 A qualitative and semi-qualitative serological test is performed for the Antinuclear Antibodies in serum or plasma. An antinuclear antibody test looks for autoimmune diseases such as lupus or rheumatoid arthritis.

Antinuclear antibodies (ANA) are a group of auto-antibodies produced by a person's immune system when it fails to adequately distinguish between "self" and "nonself." The ANA test detects these auto-antibodies in the blood. ANA react with components of the body's own healthy cells and cause signs and symptoms such as tissue and organ inflammation, joint and muscle pain, and fatigue. ANA specifically target substances found in the nucleus of a cell, hence the name "antinuclear."

ANA latex Test provides a suspension of polystyrene latex particles which has been coated with systemic lupus Deoxyribonucleoprotien (DNP) ANA produced in SLE bind to the DNP coating the latex particles. This binding is evident by rapid agglutination of the latex.


Procedure

• Allow the samples and reagents to reach room temperature.

• Place 30μl of the sample.

• Place 30μl of the reagent on slide, and mix it with stirrer and spread it in small circle, mix by rotating for 3 minutes.

• Check the result within 3 minutes


Result

If the agglutination found that means test the result is Positive.


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