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ASO Titer

 The ASO-latex is a slide agglutination test for the qualitative and semi-qualitative detection of anti-streptolysin O (ASO) in human serum. Latex particles coated with streptolysin O (SLO) are agglutinated when mixed with samples containing ASO.

Sterptolysin O is a toxic immunogenic exoenzyme produced by beta hemolytic streptoccoci of groups A, C and G. Measuring the ASO antibodies are useful for the diagnostic of rheumatoid fever, acute glomerulonephritis and streptococcal infections. Rheumatic fever is an inflammatory disease affecting connective tissues from several parts of human body as (skin, heart, joints, etc…) and acute glomerulonephritis is a renal infection that affects mainly to renal glommerulus.


Procedure

• Allow the samples and reagents to reach room temperature.

• Place 30-40μl of the sample.

• Place 30-40μl of the reagent on slide, and mix it with stirrer and spread it in small circle, mix by rotating for 2 minutes.

• Check the result within 2 minutes.

If agglutination occurs means result is positive, then we will make a dilution

• 1:1    (Serum 40μl & Normal Saline 40μl)

• 1:2    (Serum 40μl & Normal Saline 80μl)

• 1:3    (Serum 40μl & Normal Saline 120μl)


Result

• 1:1 Agglutination indicates 400 IU/L

• 1:2 Agglutination indicates 600 IU/L

• 1:3 Agglutination indicates 800 IU/L


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