Theory And Principle
Erythrocyte sedimentation rate is a non-specific test it is raised in a wide range of infectious inflammatory degenerative and malignant condition associated with change in plasma protein particularly increase in fibrinogen immunoglubins and C-reactive protein. The ESR is also affected by many other factors including anemia, pregnancy, haemoglobinopathies, haemoconcentration and treatment with anti-inflammatory drugs.
Procedure (Westergren method)
• Sample received in lavender-top tube
• Add 1.6 ml of blood in tube
• And fill 0.4 ml of sodium citrate in same tube
• Place the Westergren pipette vertically in tube
• Using the safe suction method draw the blood to Zero mark of the Westergren tubes avoid from air bubbles
• Set the timer for one hour
• After one hour take a result
Result
Read the level at which the plasma meets to the red blood cells in mm/hour. Its normal range is 0-15 mm/hour.
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