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Erythrocyte Sedimentation Rate procedure ( ESR )

 Theory And Principle

Erythrocyte sedimentation rate is a non-specific test it is raised in a wide range of infectious inflammatory degenerative and malignant condition associated with change in plasma protein particularly increase in fibrinogen immunoglubins and C-reactive protein. The ESR is also affected by many other factors including anemia, pregnancy, haemoglobinopathies, haemoconcentration and treatment with anti-inflammatory drugs.

                  

Procedure  (Westergren method)

Sample received in lavender-top tube

Add 1.6 ml of blood in tube

And fill 0.4 ml of sodium citrate in same tube

Place the Westergren pipette vertically in tube

Using the safe suction method draw the blood to Zero mark of the Westergren tubes avoid from air bubbles

Set the timer for one hour

After one hour take a result 

Result

Read the level at which the plasma meets to the red blood cells in mm/hour. Its normal range is 0-15 mm/hour.


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