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Typhoid Test ( Device Test, Device Method)

Typhoid IgG/IgM Rapid Test is performed to observe typhoid fever or also known as enteric fever. Typhoid fever is caused by salmonella typhi, a negative bacterium. About 17 million cases and 600,000 deaths occur annually in worldwide. Patients who infected with HIV have suffered from increased risk of clinical infection. This infection is caused by contaminated food and water. S.typhi enters through mouth into intestine then into bloodstream, tissues, organs. The symptoms are normally occurs between 6-30 days, main symptoms are fever and rash, other symptoms can be abdominal pain, headaches, diarrhea and vomiting.

The clinical diagnosis of typhoid fever depends on the isolation of S.typhi from blood, bone marrow or anatomic lesion. The Typhoid IgG/IgM Rapid Test is a simple and rapid laboratory test that easily detect and differentiates IgG and IgM to Salmonella Typhi, if IgM or both IgM/IgG positive suggest the current infection, while IgG positive suggests late, previous or latent infection.

The Typhoid IgG/IgM Rapid Test is lateral flow chromatographic immunoassay. The test cassette consist of :1) burgundy colored conjugate pad containing recombinant Hantigen and O antigen conjugated with colloidal gold(ho conjugates) and rabbit IgG-gold conjugates, 2) a nitrocellulose membrane strip containing two test bands (G and M bands) and a control band (C band).The M band is pre-coated with monoclonal anti-human IgM for the detection of IgM anti-S. typhi, G band is pre-coated with reagents for the detection of IgG anti-S. typhi, and the C band is pre-coated with goat anti rabbit IgG.If IgM antibodies is present in patient specimen will bind to HO conjugates, in result M band will be colored. The presence of IgG antibodies in patient specimen will bind to Ho conjugates, in result G band will colored.

                      

Procedure

• Bring sample (plasma or serum) and test components to room temperature. 

• Centrifuge the sample to separate plasma/serum from whole blood.

• Take out the device and label it with patient ID number. 

• Fill 30-40μl of specimen in dropper/micropipette and it into device.

• Fill 30-40μl of typhi dot reagent with dropper/micropipette and discard it into device. 

• Observe the result within 15 minutes.


Result

Wait for 15 minutes. Then observe the result within 15 minutes. The presence of C band means result acceptable, the presence IgM or both IgM/IgG positive suggest the current infection, while IgG positive suggests late, previous or latent infection of typhoid fever. Absence of both G and M bands leads to negative result.


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