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Widal Test and its Procedure


The widdal test is a serological technique which performed to observe for the presence of salmonella antibodies in patient serum. The Widdal test is one method that may be used to help make a presumptive diagnosis of enteric fever, also known as typhoid fever, Enteric fever is a life-threatening illness caused by infection with the bacterium Salmonella enterica serotype Typhi (S. typhi), usually transmitted through food and drinks contaminated with fecal matter. It is associated with symptoms that include high fever, fatigue, headache, abdominal pain, diarrhea or constipation, weight loss, and a rash known as "rose spots."This test is performed with TO, TH, AH, BH. If agglutination occurs at TH means result is positive but if agglutination occurs in other three spots means result is negative.  


Procedure

• Bring the specimen and components at room temperature.

• Pour 20μl of serum at slide at four different spots.

• Now pour 1 drop of each reagent in different spots of serum (TO, TH, AH, BH).

• Mix it gently, and rotate it for 3 minutes.

• Check the result within 3 minutes.


Result

If agglutination occurs at TH means result is positive but if agglutination occurs in other three spots means result is negative.

If agglutination occurs means result is positive, then we will make a dilution

• 1:1    (Serum 20μl & Normal Saline 20μl)

• 1:2    (Serum 20μl & Normal Saline 40μl)

• 1:3    (Serum 20μl & Normal Saline 60μl)

Result

• 1:1 Agglutination indicates   1:80 

• 1:2 Agglutination indicates   1:160

• 1:3 Agglutination indicates   1:320 


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